Radiant™ Taq DNA Polymerase, 5u/µl, 500 / 2,500/ 10,000 units w/ separate tube 10X PCR buffer and MgCl2
High-yields with amplicons up to 5 Kb with standard or fast cycling.
New-generation PCR buffer formulation including enhancers for maximum PCR efficiency and reaction speed
Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences.
Supplied with 10X Reaction Buffer and separate MgCl2 for flexibility and optimization.
RadiantTM Taq DNA Polymerase is a highly purified, high performance DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM Taq DNA Polymerase exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM Taq DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation buffer system which provides exceptional sensitivity
RadiantTM Taq DNA Polymerase is shipped on blue or dry ice and should be stored at –20°C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, RadiantTM Taq DNA Polymerase is stable for 12 months from date of receipt. The Kit may also be stored at 4°C for 1 month.
RadiantTM 10x Taq Reaction Buffer: The 10x Taq reaction buffer contains proprietary PCR enhancers and has been optimized for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional PCR enhancers.
Template: For complex genomic DNA, we suggest the use of 5ng - 500ng per reaction; For cDNA or plasmid DNA, please use < 100ng per reaction.
Primers: Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings (http://frodo.wi.mit.edu/primer3/). The final primer concentration in the reaction should be between 0.2μM and 0.6μM.
Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest
a 55°C annealing temperature. Increase in 2°C increments if non-specific products are present.
Extension: Optimal extension is achieved at 72°C. The optimal extension time is dependent on amplicon length and complexity. 15 seconds per kilobase(Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA. For shorter amplicons, a 1 second extension is sufficient.