Making TAE for your DNA gels has never been easier! These powder pouches are measured to make 1 Liter of 1X buffer. Just mix with water!
TAE buffer is a nucleic acid electrophoresis buffer widely used in biology labs. It is mainly used for the agarose gel electrophoresis of DNA. The main components of TAE buffer are Tris-acetate and EDTA. The final concentration of the 1X working solution is 40 mM Tris-acetate and 1 mM EDTA.
TAE buffer works particularly well for separating DNA molecules larger than 13 kb in size. Also, it is recommended to use TAE buffer for recovering DNA from the gel after electrophoresis.
QuickSilver™ TAE is supplied in the form of white granule powder that instantly dissolves in water. One pouch conveniently makes 1 L of 1X TAE working solution.
l Migration rates of superhelical DNA molecules proportional to their molecular weights.
l Ideal for separating DNA molecules bigger than 13 kb in size.
l Recommended when recovering DNA fragments from the gel.
l Not recommended for overnight electrophoresis.
To make 1 L of 1X TAE:
1. Set up a magnetic stirrer with stir bar in a suitable mixing beaker or bottle and add 600 ml distilled water.
2. While stirring, slowly pour the whole contents from 1 pouch of TAE into the beaker; wait until all of the powder is dissolved.
3. Add distilled water to bring the volume to 1 L.
100 pouches per case. Costs $1.48 per liter of TAE buffer!