YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer.
YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgGbinding proteins can be immuno-detected on film or by CCD imaging.
YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems.
In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa).
The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
The YesBlot™ Western Marker I contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer.
250 µl 5 µl per well for 50 applications
4°C ≥ 3 months
-20°C ≥ 24 months