Nucleic Acid Labeling & Detection

Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA, ssDNA and RNA using an instrument such as the Qubit™, SmartDrop™ XF, or Accuris™ Fluorometer.

The reagents are highly selective for double-stranded DNA (dsDNA), so quantification results will not be skewed by single-stranded DNA (ssDNA), RNA, protein, or nucleotides in a sample. 

Included with each kit are a concentrated fluorescent assay reagent, dilution buffer, and ready-to-use DNA standards.  The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL), and the concentration can be determined using an appropriate fluorometer instrument that utilizes blue excitation and measures green fluorescence.

High Sensitivity dsDNA Assay Kit

The Accuris High Sensitivity Kit provides an accurate method for quantitation of dsDNA samples of low concentration.  Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005–120 ng/µl, providing a detection range of 0.1–120 ng.

Broad Range dsDNA Assay Kit

The Accuris Broad Range Kit provides an accurate method for quantitation of dsDNA in samples that have a wide concentration range starting at 0.2 ng/µL .

Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng.

ssDNA Quantification Kit

The Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents

RNA High Sensitivity Quantification Kit

The Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration.

This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL

RNA Broad Range Quantification Kit

The Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification.

This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL.

This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents