Consistently extract high-yield DNA from a comprehensive range of forensic samples including saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair).
The process is uniquely effective for both small-volume and trace samples.
ForensicGEM is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA.
Extracted DNA can be used for many types of genotyping including SNP and STR analysis as well as quantitative, multiplex and end- point PCR.
Why is Exymes Forensic GEM nucleic acid extraction the best choice for DNA extraction?
Because there is no concentration step in the procedure and so the concentration of the extract is dependent on: 1) The quality of the sample; 2) In the case of swabs, the type of swab and the volume of water used to wash the swab; 3) The extraction volume (which in some cases can be scaled).
Exymes ForensicGEM delivers superior DNA for demanding forensic analysis:
The method produces double-stranded DNA, and STR profiling, PCR, and qPCR all require dsDNA. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.
Exymes ForensicGEM uses a closed-tube workflow built for speed and sample security:
Transform your lab's efficiency with a fully hands-off, single-tube workflow. From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity.
This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.
These kits are for use with your current laboratory thermal cycler.
Extraction from a vairety of forensic blood samples including:
- Liquid (fresh, EDTA, Heparin, Citrate)
- Swab
- Stains
- Storage Card (FTA)
Tissue samples, including:
- Animal (scrappings, fat and muscle)
- Tape lifts
- Hair
Saliva samples including:
- Liquid
- Swab
- Stains
- Storage Card (FTA
DNA extracted using forensicGEM is largely single-stranded because of the 95°C heat step.
For accurate yield assessment, a qCR is recommended. If standard fluorescent chelating dyes are to be used for normalizing samples, then we recommend taking a sample of the extract before the 95°C step.
Alternatively, you can generate a standard curve using a previously made extract that has been quantified.
Click on the icon below to view the protocol for this nucleic acid extraction chemistry:
