The primers and probes are selected from the regions of RNase P, ORF1ab and E gene.
Two primer/probe sets are designed for specific detection of SARS-CoV-2. An additional primer/probe set to detect human RNase P gene (as an internal control) is also included.
RNA extracted from respiratory specimens is reverse transcribed and amplified in a Real-Time PCR Thermocycler..
The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to target sequence between the forward and the reverse primers.
During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.
The SARS-CoV-2 Real-Time RT-PCR Assay also contains a positive control (Plasmid DNA) and a negative control for testing process monitoring.
Click here to see a list of everything included in the kit and for complete protocols.
