The ViraTrapTM retrovirus mini purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant.
Up to 3 x1012 viral particles can be purified from cell culture media of 1 to 2 T75 flasks.
Traditionally the recombinant retrovirus is purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same retrovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Materials Required But Not Supplied
1. ddH2O
2. PBS
3. 0.45 μm and 0.22 μm filters
4. Rack holder for columns
