Nanopatterned Cultureware

NanoSurface Biomedical’s ANFS (Anisotropically NanoFabricated Substratum) technology brings an in vivo-like cell culture platform to customers for in vitro cell/tissue studies. Featuring topographical resemblance to the extracellular matrix, our nanopatterned substrates provide a topographical and mechanical niche for cells to grow as if they are in vivo.

Our nanopatterned surfaces influence both cellular morphology and phenotype. For example, when used with cardiomyocytes, we observe formation of striated sarcomeres, intercalated discs, and polarized expression of gap junction proteins such as cinnexin43.

Phenotypically, cells cultured on ANFS are more mature, and more physiology, so that your studies are more predictive. 


  • Surfaces with an alternative repetition of nanoscale ridges and grooves

  • Plasma-treated for enhanced cell attachment (untreated also available for your own surface treatments such as Poly-L Lysine, Fibronectin, Collagen, etc.)

  • Biocompatible polymeric material

  • Minimally auto-fluorescent material

  • Ideal for confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations

  • Optimal nanopattern dimensions for maturation and differentiation of many cell types 


  • Research studies on many types of cells and tissues in vitro

  • Disease models for understanding diseases and developing cures

  • Drug screening (e.g. in vitro cardiac tissues for testing cardiotoxicity)